p irs 1 ser307  (Cell Signaling Technology Inc)

Journal: Antioxidants

Article Title: 14-Deoxy-11,12-didehydroandrographolide Alleviates IL-1β-Induced Insulin Resistance by Modulating NOX2-Driven ROS Generation and Restoring Insulin Signaling in 3T3-L1 Adipocytes

doi: 10.3390/antiox14101155

Figure Lengend Snippet: IL-1β induces IRS-1 (Ser307) phosphorylation through ERK, JNK, and IKK pathways. ( A ) Cells were treated with IL-1β (10 ng/mL) for 0, 10, 30, and 120 min. ( B – D ) Cells were pretreated with PD98059 (15 μM), SP600125 (10 μM), or Bay11-7082 (20 μM) for 2 h, followed by stimulation with IL-1β (10 ng/mL) for 10 min. Protein levels of p-ERK, p-JNK, p-IKK, p-IRS-1 (Ser307), and α-actin were analyzed by Western blotting. Data are expressed as mean ± SD from three independent experiments. Values not sharing the same letter are significantly different ( p < 0.05).

Article Snippet: Membranes were blocked with 5% nonfat dry milk at room temperature for 1 h and then incubated overnight at 4 °C with primary antibodies: anti–p-IRS-1 (Tyr608) (Cat# 09-432, Millipore, St. Louis, MO, USA; 1:1000), anti–p-IRS-1 (Ser307) (Cat# 2381, Cell Signaling Technology, Danvers, MA, USA; 1:1000), anti–p-AKT (Thr308) (Cat# 4056, Cell Signaling Technology; 1:1000), anti–p-ERK1/2 (Cat# 9101, Cell Signaling Technology; 1:1000), anti–p-JNK (Cat# 4668, Cell Signaling Technology; 1:1000), anti–p-IKK (Cat# 2697, Cell Signaling Technology; 1:1000), anti–p67phox (Cat# 3923, Cell Signaling Technology; 1:1000), anti–Na + /K + -ATPase (Cat# 3010, Cell Signaling Technology; 1:1000), and anti–α-actin (Cat# A2066, Sigma-Aldrich, St. Louis, MO, USA; 1:5000).

Techniques: Phospho-proteomics, Western Blot

Journal: Antioxidants

Article Title: 14-Deoxy-11,12-didehydroandrographolide Alleviates IL-1β-Induced Insulin Resistance by Modulating NOX2-Driven ROS Generation and Restoring Insulin Signaling in 3T3-L1 Adipocytes

doi: 10.3390/antiox14101155

Figure Lengend Snippet: deAND suppresses IL-1β-induced IRS-1 (Ser307) phosphorylation by inhibiting ERK and JNK activation. Cells were pretreated with deAND (5 or 10 μM) for 16 h and then exposed to IL-1β (10 ng/mL) for 10 min. Protein levels of p-ERK, p-JNK, p-IKK, p-IRS-1 (Ser307), and α-actin were analyzed by Western blotting. Data are presented as mean ± SD of three independent experiments. Values not sharing the same letter differ significantly ( p < 0.05).

Article Snippet: Membranes were blocked with 5% nonfat dry milk at room temperature for 1 h and then incubated overnight at 4 °C with primary antibodies: anti–p-IRS-1 (Tyr608) (Cat# 09-432, Millipore, St. Louis, MO, USA; 1:1000), anti–p-IRS-1 (Ser307) (Cat# 2381, Cell Signaling Technology, Danvers, MA, USA; 1:1000), anti–p-AKT (Thr308) (Cat# 4056, Cell Signaling Technology; 1:1000), anti–p-ERK1/2 (Cat# 9101, Cell Signaling Technology; 1:1000), anti–p-JNK (Cat# 4668, Cell Signaling Technology; 1:1000), anti–p-IKK (Cat# 2697, Cell Signaling Technology; 1:1000), anti–p67phox (Cat# 3923, Cell Signaling Technology; 1:1000), anti–Na + /K + -ATPase (Cat# 3010, Cell Signaling Technology; 1:1000), and anti–α-actin (Cat# A2066, Sigma-Aldrich, St. Louis, MO, USA; 1:5000).

Techniques: Phospho-proteomics, Activation Assay, Western Blot

Journal: Cell Discovery

Article Title: A decrease in Flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders

doi: 10.1038/s41421-025-00789-x

Figure Lengend Snippet: Key materials and software.

Article Snippet: Primary antibody against p-IR-β , Cell Signaling Technology, Inc., Shanghai, China , #2381.

Techniques: Software, Recombinant, Luciferase, Reporter Gene Assay, Plasmid Preparation, Control, Transfection, Enzyme-linked Immunosorbent Assay, Staining